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A framework for assessing confidence in metabarcoding assays and results

发布时间	所属地区	所属机构	语言
2024年08月20日	--	--	英语 (English) 中文

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Published date: 20 August 2024

Open opportunity - This means that the contract is currently active, and the buying department is looking for potential suppliers to fulfil the contract.

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Contract summary

Industry

Research services - 73110000

Location of contract

United Kingdom

Value of contract

£1 to £33,000

Procurement reference

NE200824FACMAR

Published date

20 August 2024

Closing date

13 September 2024

Closing time

12pm

Contract start date

2 October 2024

Contract end date

28 February 2025

Contract type

Service contract

Procedure type

Open procedure (below threshold)

What is an open procedure (below threshold)?

Any interested supplier may submit a tender in response to an opportunity notice.

This procedure can be used for procurements below the relevant contract value threshold.

Contract is suitable for SMEs?

Yes

Contract is suitable for VCSEs?

Description

DNA-based methods have the potential to significantly change how we monitor and assess ecosystems. These methods are now being used more widely within Natural England, particularly the detection of ecological communities using environmental DNA (eDNA). This is often conducted by employing metabarcoding assays which target specific species assemblages (e.g. fish, mammals) or broader taxonomic groups (e.g. vertebrates, invertebrates, bacteria). It is difficult for Natural England staff to understand, interpret, and determine confidence in the results of different metabarcoding assays or assess the risk of false positive or false negative results.

This project aims to address some of these challenges through the development of an eDNA metabarcoding assay validation framework. This will include: establishing key criteria to enable the classification of metabarcoding assays based on their taxonomic coverage, taxonomic resolution and detection sensitivity for different groups; and assessing the suitability of metabarcoding assays for different sample types and environments. This will enable end-users to determine the recommended scenarios for application of a given assay and improve assay performance with further validation.

More information

Attachments: 2024-08-20_RFQ_metabarcoding_validation.doc

Bidding documents

RFQ; 2024-08-20_Order_Form_NE_Standard_Goods_Services_Terms_and_Conditions.docx

Contract draft; LIT63284_Commercial_Response_Basic.docx

Evaluation report
Additional text: To apply for this opportunity you must submit your quotation meeting the requirements detailed in the Request for Quotation (RFQ) attached.
Your response must be sent to monitoring.innovation@naturalengland.org.uk by 12 midday on 13th September 2024.

If you have any clarification questions linked to this opportunity or the procurement process please submit these via email to monitoring.innovation@naturalengland.org.uk. Please note that, unless commercially sensitive, both the question and the response will be circulated to all tenderers.